
13.07.2019 A new approach to the pre-analytical phase of venous plasma glucose analysis
One of the co-authors of intervactechnology test tube for pregnant women took part in the congress IFCC-EFLM European Congress of Clinical Chemistry and Laboratory Medicine in Barcelona.
Dorofeykov Vladimir: Lesgaft National State University of Physical Culture, Sport and Health, St. Petersburg, Russia
The prevalence of diabetes mellitus, glucose tolerance disorders and especially gestational diabetes mellitus (GDM) is growing.
The need for the diagnosis and treatment of GDM, based on the accurate determination of glucose in plasma of venous blood by laboratories, has identified.
AIM: to test two new types of vacuum systems containing separation gel and sodium fluoride.


MATERIAL & METHODS
IntervacTechnology (Estonia) has developed two types of vacuum systems containing separation gel and sodium fluoride:
• with ethylendiaminetetraacetic (EDTA) or
• sodium oxalate as the anticoagulant.
Comparative tests of both types of vacuum systems were carried out in comparison with the basic systems without separating gel.
1. The degree of hemolysis was determined by “HemoCue Plasma / Low Hb” analyzer (Sweden).
2. Glucose determination was carried out using an
automatic analyzer “Architect c8000” (Abbott) immediately after blood centrifugation, not later than 60 minutes after blood sampling and 24 hours of storage of samples at room temperature.
3. Glucose levels were determined in 16 donors in 3 types of vacuum systems 1 hr and 24 hrs after blood collection.
RESULTS
> Vacuum systems containing oxalate and separation gel had lower hemolysis rates than the ones with EDTA.
> The results of glucose determination in vacuum systems stored in the refrigerator and at room temperature did not show a statistically significant difference.
> The choice of anticoagulant did not affect the results of glucose determination during storage of samples for 24 hours.
> Vacuum systems with a separation gel and anticoagulant showed consistent results on the filling of blood to the necessary amount in all tests.
> The level of glucose in vacuum systems with separating gel and fluoride in an hour and after 24 hours did not differ significantly from the initial level of glucose in the standard sample tubes.
> It is necessary to indicate the possibility of hemolysis visible to the eye when using a new type of vacuum systems that does not affect the accuracy of determining the concentration of glucose in the blood plasma of patients.
CONCLUSION
Vacuum systems with separating gel, sodium oxalate and fluoride are the optimal solution for centralized laboratories, where samples are delivered by transport within 2 to 24 hours.